The transparent hot stage TP-R200-M of the upright microscope has high cost-effectiveness and practicality; Customization can be made according to actual needs to better meet customer needs. When observing live cells and temperature sensitive organisms, there is no need to worry about the inconvenience of observation and research caused by external factors leading to cell or organism death. The application in fields such as cell engineering, neuroscience, and genetic engineering will have a very wide range of prospects. It not only provides the required temperature guarantee, but also has an automatic insulation and constant temperature function, which can provide a more comfortable environment for the observed samples, but also provide more ample observation time and observation methods for observers